The systemic effects of experi, emtally induced periodontitis in rats

近年来，有证据表明牙周病患者血清中抗牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）抗体滴度、胆固醇、甘油三酯和血糖水平均显著高于健康人。提示牙周病可影响糖脂代谢。为探讨实验性牙周炎对大鼠血清甘油三酯、血糖、肿瘤坏死因子- α（TNF- α）、白细胞介素-6（IL-6）、C-反应蛋白（CRP）和脂联素（adiponectin）的影响，将48只雄性Wistar大鼠分为实验性牙周炎组和对照组。在感染组的下颌第一磨牙周围施加一个小的弹性环来诱导实验性牙周炎。1周后，将浸有Pg的丝线插入Pg感染组大鼠的牙周袋中，并在M1周围用弹性环固定。四周后，通过从心脏抽血处死大鼠并取出下颌骨。对照组不治疗，维持5周，感染组牙槽骨丢失较对照组明显。然而，高蔗糖饲料喂养的大鼠和高脂肪饲料喂养的大鼠之间没有显著差异。至于血清甘油三酯和葡萄糖的水平，有没有明显的差异之间的Pg感染和对照组。感染组牙周组织TNF-α、IL-6、CRP和脂联素水平均低于对照组，但差异无统计学意义。

In recent years, some evidence has been presented that serum antibody titer against Porphyromonas gingivalis (Pg), cholesterol, triglyceride and blood glucose level were significantly higher in patients with periodontal disease than in healthy subjects. Thus, it is suggested that periodontal disease could influence the metabolism of lipids and glucose. This study examined the effects of experimentally induced periodontitis on the serum level of triglyceride, glucose, TNF- α, IL-6, C-reactive protein (CRP) and adiponectin in rats.Forty-eight male Wistar rats fed high-sucrose or high-fat diet were divided into Pg-infected groups or control groups. Experimental periodontitis was induced by applying a small elastic ring around the mandibular first molar (Ml) in Pg-infected groups. After one week, Pg soaked silk sutures were inserted into periodontal pockets and fixed by elastic rings around M1 of the rats in the Pg-infected groups. After four weeks, rats were sacrificed by drawing blood from the hearts and removed mandibular bones. Rats of control groups were not treated and maintained for 5 weeks.Significant alveolar bone loss was found in the Pg-infected groups compared with that in the control groups. However, there was no significant difference between rats fed high-sucrose diet and those fed high-fat diet. With regard to the serum levels of triglyceride and glucose, there were no significant differences between Pg-infected and control groups. All of the TNF-α, IL-6, CRP and adiponectin levels of the Pg-infected group were under those of the control level, although there were no significant differences.Further studies are needed to determine the effects of diet on the systemic response to Pg infection of the periodontium.