STUDY ON ANTISEPTIC EFFECT OF SILVER AND COPPER IONS AGAINST LEGIONELLA IN HOT SPRING WATERS

1.研究了温泉化学成分对银离子防腐效果的影响：(1)银离子防腐效果随温泉中氯离子和硫酸离子含量的增加而延迟。(2)有些温泉虽然有足够的银离子浓度，但没有显示出防腐效果。其原因将归因于银离子与某种温泉成分形成络合物。本文研究了银离子对阿米巴和阿米巴中军团菌细胞的杀菌作用：(1)浓度为0.05ppm的银离子对阿米巴和阿米巴中的军团菌细胞无杀灭作用，而2.5ppm的银离子对阿米巴中的军团菌有杀菌作用。(2)包囊阿米巴中军团菌细胞即使在2.5ppm浓度下也不能被杀死，但在5ppm浓度下仍能被杀死。(3)-5ppm浓度的银离子对阿米巴细胞本身无明显的杀伤作用。水溶液中的铜离子浓度随着pH的升高而降低。在本研究中，…将包铜颗粒分别加入不同pH值的水溶液中，最终浓度分别为0.1%和0.5%。洗脱液中铜的浓度在0.1%和0.5%范围内有所不同，但不同pH值对铜的溶解速率随时间变化不大。4.研究了铜离子对阿米巴和阿米巴单独作用下军团菌细胞的防腐作用：(1)1ppm的铜离子对滋养体军团菌细胞无防腐通道，而5ppm和10ppm的铜离子对军团菌细胞的杀灭率分别为76%和95%。(2)1ppm的铜离子对囊状军团菌细胞和滋养体细胞均无杀灭作用。浓度为5ppm和10ppm时，对军团菌细胞的杀灭率分别为85%和94%。(3)在铜离子浓度为5ppm和10ppm时，阿米巴细胞存活率分别为74%和47%。在pH值为7.2的铜离子溶液中对军团菌的杀菌效果实验中，加入0.5ppm的铜离子后6h，军团菌细胞被完全杀灭。另一方面，每0.1%的铜溶液进行调整。PH值至6.0、7.4和8.5均表现出防腐效果，蒸馏水、自来水和海水在铜浓度为0.1%时也有相同的防腐效果。由于温泉的pH值和化学成分不同，温泉水对军团菌的杀菌效果不同。较少

1. We studied the influence of chemical composition of hot springs to the antiseptic effect of silver ion.(1) The antiseptic effect of silver ion delayed according to-increases of chloriide and sulfuric acid ions in hot spring. (2) Some hot spring did not show any antiseptic effect despite enough silver ion concentration. The cause will be attributed to that silver ion formed complex with some kind of hot spring components.2. Antiseptic effect of silver ion against legionella cells in amoeba or amoeba alone was studied.(1) While legionella cells in amoeba were not killed at the concentration of 0.05 ppm of silver ion, silver ion of 2.5ppm showed an antiseptic effect. (2) Legionella cells in cyst encysted amoeba were not killed even at the concentration of 2.5ppm but did at the concentration of 5ppm. (3) Amoeba cells themselves were not killed by the concentration of-5ppm of silver ion.3. The concentration of copper ion in an aqueous solution decreased with a rise in pH.In this study … More , particles coated with copper were added into each aqueous solution with different pH at a final concentration of 0.1%and 0.5%, respectively. Eluted copper concentration was different in 0.1% and 0.5%, however there was no difference among different pH in dissolution rate of copper along with the time course. 4. Antiseptic effect of copper ion against legionella cells in amoeba or amoeba alone was studied.(1) The antiseptic duct of copper ion against legionella cells m trophozoite cells was not shown at the concentration of 1ppm, while legionella cells of 76% at 5ppm and 95% at 10ppm were killed, respectively. (2) The legionella cell in cyst form were not killed at the concentration of 1ppm of copper ion as well as in trophozoite cell. Eighty fie % and 94% of legionella cells were killed at the concentration of 5ppm and 10ppm, respectively. (3) Seventy four % and 47% of amoeba cells survived in the copper ion concentration of 5ppm and 10ppm, respectively.5. In the experiment of antiseptic effect against legionella in copper ion solution with pH7.2, Legionella cells were completely killed at 6h after the addition of 0.5 ppm copper ion. On the other hand, each 0.1% copper solution adjusted. to pH6.0, 7.4 and 8.5 showed all antiseptic effect, and distilled water, tap water and marine water adjusted to copper concentration of 0.1% had also the same effect. The antiseptic effect of hot spring waters against legionella was diversed among each hot spring due to the differences of pH and chemical composition of hot springs. Less