THE INFLUENCE OF PH AND THE ELUCIDATION OF CONTAMINATION ROUTE FOR LEGIONELLA IN HOT SPRING WATERS

1.本文分析了各温泉浴场室内、室外浴缸水氯化消毒后水质的变化，并进行了军团菌的流行病学调查。在这些水中进行。2.过渡到VNC在不同pH和温度条件下，研究了微宇宙中嗜肺军团菌（L.pneumophila）种群的存活期（活的但不可培养的）。（1）在极低pH的微宇宙中，嗜肺军团菌的整个细胞在短时间内被杀死，而在高pH的微宇宙中，细胞数量逐渐减少。提示pH 5的微宇宙中细胞群向VNC期转移。（2）嗜肺军团菌能在pH6.0 ~ 8.2或25 ~ 42℃的微宇宙中长期存活。（3）嗜肺军团菌不能在45℃的高温水中存活，但本实验结果表明，嗜肺军团菌在45℃的高温水中仍能以VNC的形式存活。然而，嗜肺军团菌是q ...更多信息 在50摄氏度以上的环境中迅速死亡。3.研究了氯对嗜肺军团菌和阿米巴的防腐效果。（1）嗜肺军团菌在微环境中能在0.4ppm余氯下存活1 min以上。在1.0ppm的余氯中，生物体很快被杀死。（2）将阿米巴细胞加入到微宇宙中，嗜肺军团菌在阿米巴细胞吞噬体中增殖并吞噬细胞，并在孵育过程中重复上述过程。孵育24小时后，将各种浓度的氯加入到微宇宙中。即使在用50 ppm的余氯消毒的微宇宙中也检测到少量的肺炎链球菌细胞。40%的阿米巴细胞生活在10 ppm余氯的微宇宙中。这些细胞可能以胞囊细胞的形式存在，对氯具有抵抗力，因此，这些胞囊中的肺炎军团菌细胞也受到保护，不受氯的杀菌作用。（1）采用随机扩增多态性DNA（RAPD）技术对从温泉及其周围土壤中分离的血清型相同的嗜肺军团菌进行了基因分型。到目前为止，这些隔离物的遗传特性没有得到承认。（2）对从带有循环系统的温泉浴场的浴缸和滤水池中分离的多种血清型嗜肺军团菌进行RAPD分析。不同血清型的分离株之间存在遗传一致性。通过脉冲场凝胶电泳（PFGE）进行进一步确认。少

1.Change in the quality due to chloridizing against sampled water collected from indoor or outdoor bathtub of various spa was analyzed and epidemiological study of Legionella spp. in these water was carried out.2.Transition to VNC (viable but not cultivable) stage of L.pneumophila population in microcosms preparated in sampled water was examined under conditions of different pH and temperature(1)Whole cells of L.pneumophila were killed in a short period in microcosm with extreme low pH, while cell numbers were gradually reduced in microcosm with high pH. On the other hand, it was suggested that cell population in microcosm with pH5 shifted to VNC stage. (2)L.pneumophila could survive for a long period in microcosms in a range of pH6.0 to 8.2 or 25 to 42℃ (3)It has previously been thought that L.pneumophila could not survive in high-temperature water of 45℃ however, the present data suggested that L.pneumophila could survive as VNC even under this condition. However, L.pneumophila was q … More uickly killed in microcosms with temperature of 50 or more degrees Celsius.3.Antiseptic effect of chlorine against L.pneumophila and Acanthamoeba was examined.(1)L.pneumophila could survive for one minute or more in microcosm with 0.4 ppm of residual chlorine. In residual chlorine of 1.0 ppm, the organisms were quickly killed. (2)Acanthamoeba cells were added into microcosms, and then L.pneumophila multiplicated in phagosomes of amoeba cells and ate away cells, and repeated these process during incubation. After incubation for 24 hrs, a various concentrations of chlorine were added into microcosms. A small amount of L.pneumonia cells was detected even in microcosm disinfected with 50 ppm of residual chlorine. Forty % of Acanthamoeba cells was living in microcosm with 10 ppm of residual chlorine. These cells might exsist as cyst cell having resistance to chlorine, therefore, L.pneumonia cells were also protected in these cysts from bactericidal effect of chlorine.4.The route that Legionella contaminate spa was studied.(1)Genotype of L.pneumophila isolates with identical serotype isolated from spa and the surrounding soil was determined by randomly amplified polymorphic DNA analysis(RAPD). As so far examined, genetic identity among these isoltes was not recognized. (2)RAPD analysis was done about L.pneumophila isolates of a variety of serotype isolated from bathtub and filtlate tank in spa with circulating system. Genetic identity was shown among isolates of different serotype. Further confirmation by pulse field gell electrophoresis(PFGE) was carried out. Less