confocal laser-scanning microscope

这是一项关于安装在贾斯图斯-利比格大学吉森医学院解剖和细胞生物学研究所(FB11)的中央成像单元的直立配置的共焦激光扫描显微镜的建议。它将取代2003年购买的共焦激光扫描显微镜，该显微镜不再提供维护，不符合申请工作组当今最先进研究项目的要求。它将由解剖学和细胞生物学研究所的中央成像部门运营，该研究所也为所有教职员工提供服务。用户的研究项目涵盖了细胞生物学的广泛主题，包括刷状细胞中的信号转导和气管、胆囊和尿路中的刷状细胞效应器功能，男性生殖道中的免疫细胞相互作用，组织特有的过氧化物体的分子组成和功能，以及cGMP依赖的信号和平滑肌的作用，尤其是男性生殖道。所有这些项目都对荧光显微成像有很高的需求，可以在高空间分辨率下分辨多种荧光染料，并允许消除背景荧光，这是通过最先进的共焦激光扫描显微镜实现的。这些项目需要同时对四个甚至最多七个荧光团进行成像，其中包括激发在紫外线范围内的染料，以及其他发射在远红范围内的染料。这就需要一个宽的激发范围，白光激光加紫外光二极管激光能最好地覆盖这一范围。用于快速消除背景自发荧光信号的技术也需要白光激光器。移植器官的生命成像在一些项目中起着核心作用，因此需要直立的显微镜配置，额外的浸入式物镜，以及显微镜舞台上的温控灌注室。

This is a proposal for a confocal laser scanning microscope in an upright configuration to be installed at the central imaging unit at the Institute for Anatomy and Cell Biology of the Medical Faculty (FB11) of the Justus-Liebig-University Giessen. It shall replace a confocal laser scanning microscope bought in 2003 for which maintenance is no longer been offered and which does not meet nowadays requirements of state-of-the-art research projects of the applying working groups. It will be run by the central imaging unit of the Institute for Anatomy and Cell Biology which also offers service to all members of the faculty. The research projects of the users cover a wide spectrum of topics in cell biology, including signal transduction in brush cells and brush cell effector functions in trachea, gallbladder and urethra, immune cell interactions in the male reproductive tract, tissue-specific molecular composition and function of peroxisomes, and role of cGMP-dependent signaling and smooth muscle with particular focus on the male genital tract. All these projects have in common a high need of fluorescence microscopic imaging with discrimination of multiple fluorescent dyes at high spatial resolution and allowing for elimination of background fluorescence, which is enabled by state-of-the-art confocal laser scanning microscopes. The projects require simultaneous imaging of often four and up to seven fluorophores, among them dyes with excitation in the UV range and others with emission in the far-red range. This results in the need of a wide excitation range, which will be covered best by a white light laser plus an UV diode laser. A white light laser is also needed for techniques for fast elimination of background autofluorescence signals. Life imaging of explanted organs plays a central role in some projects, resulting in the need of an upright microscope configuration, additional dip-in objective lenses, and a temperature-controlled perfusion chamber on the microscope stage.