INVESTIGATION OF FISH ROE ALLERGEN AND ITS CROSS-REACTIVITY

鲑鱼、沙鱼和鲱鱼等鱼子在日本很受欢迎，在过去的十年里，许多鱼子过敏的病例都被报道过，尤其是在儿童时期。然而，这并不是说；本研究的目的是鉴定鱼卵的过敏原，并研究不同种类鱼卵之间的IgE交叉反应性。结果如下：(1)11种鱼卵(鲑鱼：金枪鱼，库页鱼：金枪鱼，虹鲑鱼：金枪鱼，彩虹鲑鱼：金枪鱼，金枪鱼金枪鱼(Spirinchus lanceolatus Capelin：Mallotus villosus)含有的卵黄蛋白能与抗鲑鱼β‘成分的兔Ig G(抗-β)反应，也能与鲑鱼卵过敏患者的Se…反应。(2)鲑鱼鱼卵中的主要变应原为β‘-组分及其相关片段。(3)鲑鱼卵β’-组分之间存在Ig E交叉反应，杂三文鱼与黑线鳕鱼之间也存在β‘-组分的交叉反应。(5)从鲑鱼肝脏中提取出编码β‘-组分的cDNAs，其编码的氨基酸序列与直接测序法得到的序列一致。(6)从Walleye Pollack Roe公司纯化了β’-组分蛋白(Mw为17,000)，并以大西洋鳕鱼卵黄原蛋白的内部序列为模板测定了其约70%的氨基酸序列。较少

Fish roe such as salmon, walleye pollack, and herring roes are popularly consumed in Japan and much cases of fish-roe-allergy, particularly in childhood, have been reported last decade. However; there is little information on fish roe allergens.The objective of this study is to identify fish roe allergens and to investigate the IgE cross-reactivity among various kinds of fish roes.The results were as follows: (1) Eleven kinds of fish roes (Churn salmon: Oncorhynchus keta, Sakhalin taimen: Hucho perryi, rainbow trout: Oncorlhynchus mykiss Japanese char: Salvelinus leucomaenis, dolly vuuden: Salvelinus malma, walleye pollack: Theragra chalcogramma, dusky sole Lepidopsetta mochigare, Slime flounder:Microstomus achne, atka mackerel: Plecurogrammus azonus, sishamo smelt: Spirinchus lanceolatus capelin: Mallotus villosus) contained yolk protein that reacted with anti chuun-salmon-β'-component rabbit IgG (anti-β) and they also reacted with specific IgE in chum salmon roe-allergic patients' se … More ra.(2) The major allergens in salmonid fish roes were β'-component and its related fragments.(3) There were IgE cross-reactivity among salmonid roe β'-components, and the IgE cross--reactivity was also confirmed between churn salmon and walleye pollack roes.(4) Amino acid sequence of churn salmon β'-component (Mw. 16,000) was examined and the sequences of 91 residues (about 60% of the total sequence) were determined by peptide mapping using the complete sequence of rainbow trout viltellogenin.(5) cDNA encoding β'-component was obtained using mRNAs extracted firm chum salmon liver and the encoded amino acid sequence was coincided with the sequence obtained by the direct peptide mapping.(6) β'-component like protein (Mw 17,000) was purified firm walleye pollack roe, and about 70% of the total amino acid sequence was determined using an inside arrangement of Atlantic cod vitellogenin as a template.These findings would contribute understanding fish roe allergy and improvement of QOL of allergic patients. Less