Study for inhibitory regulation of auto reactive T-cell by DC-HIL

这一年我研究了树突状细胞介导的抗原特异性t细胞增殖和抑制。前一年进行了动物体内反应研究；然而，这项研究还不足以证实t细胞的特异性反应。因此，今年开发了体外分析系统（Tamura, et. al. Journal of Veterinary Medical Science 2007）。使用恶性黑色素瘤或恶性淋巴瘤制备的抗原，脉冲树突状细胞，并检测淋巴细胞的增殖。虽然载有恶性黑色素瘤制备的抗原的树突状细胞诱导淋巴细胞增殖，但载有恶性淋巴瘤制备的抗原的树突状细胞使淋巴细胞增殖失败。负载恶性黑色素瘤制备抗原的树突状细胞表达DC-HIL的水平无法检测到，而负载恶性淋巴瘤制备抗原的树突状细胞表达DC-HIL的水平较高。这些数据表明DC-HIL是一个与T细胞增殖抑制密切相关的分子。本研究认为DC-HIL控制T细胞抗原特异性抑制，其异常可能破坏T细胞对自身组织的耐受性。

I investigated dendritic cell-mediated antigen specific T-cell proliferation and suppression in this year. The reaction was studied on animals in vivo in previous year ; however, the study was not enough to validate specific reaction of T-cells. Therefore, in vitro analysis system was developed this year (Tamura, et. al. Journal of Veterinary Medical Science 2007). Using antigen prepared from malignant melanoma or malignant lymphoma, dendritic cells were pulsed and proliferation of lymphocytes were examined. Although dendritic cells loaded with antigen prepared from malignant melanoma induced proliferation of lymphocytes, dendritic cells loaded with antigen prepared from malignant lymphoma failed proliferation of lymphocytes. Dendritic cells loaded with antigen prepared from malignant melanoma express undetectable levels of DC-HIL, while that loaded with antigen prepared from malignant lymphoma showed high level expression of DC-HIL. The data suggested that DC-HIL is a molecule closely associates suppression of T cell proliferation. From this study, it was considered that DC-HIL control antigen-specific suppression of T cell and its abnormality may disrupt tolerance of T cells to self-tissues.