Prevention of spiral wave formation and breakup bycontmlof intracellular calcium dynamics

最近使用计算机模拟的理论研究表明，细胞间Can动力学在螺旋波再入的启动和维持中起着重要作用，这是室性心动过速（如室颤/心动过速）的主要机制。在本研究中，我们开发了两种类型的双光学成像系统，以同时记录实验动物离体心脏制剂的动作电位/细胞内Ca^2+荧光图像：第一种系统有一个双色镜来分离荧光信号和2个高速CMOS摄像机，第二种系统包括一个高速开关装置，激励led结合旋转滤光片用于荧光。以合理的时空分辨率记录了兔左室心外膜langendorff灌注心肌的一系列动作电位和细胞内Ca^2+的荧光图像。然而，在较稳定的系统中，需要额外开发图像处理软件来调整双荧光图像，而在后一系统中，需要提高时间分辨率（20帧/秒）以精确分析动作电位与细胞内Ca^2+动力学之间的关系，以阐明细胞内Ca^2+动力学在螺旋波再入中的作用。

Recent theoretical studies using computer simulation has suggested that intercellular Can dynamics plays an important role in the initiation and maintenance of spiral wave reentry, which is a principal mechanism underlying ventricular tachyarrhythmias such as ventricular fibrillation/tachycardia. In this study, we have developed 2 types of dual optical mapping systems to record action potential/intracellular Ca^2+ fluorescence images simultaneously from isolated heart preparations of experimental animals: the first system has a dicrotic mirror to separate fluorescence signals and 2 high-speed CMOS video cameras, and the second system includes a high-speed switching apparatus of excitation LEDs combined with rotating optical filters fir fluorescence. A series of fluorescence images for action potentials and intracellular Ca^2+ were recorded simultaneously from the left ventricular epicardial myocardium Langendorff-perfused rabbit hearts with reasonable spatiotemporal resolution. In the firmer system, however, an additional development of image processing software to adjust the dual fluorescence images will be required, and in the latter system, an improvement of time resolution (20 frames/s) will be needed for precise analysis of relationship between action potentials and intracellular Ca^2+ dynamics to elucidate the roles of intracellular Ca^2+ dynamics on spiral wave reentry.