Pathogenesis of Saunpus and roylmus assoaated bronchioitis obliterancee (SABO)

最近，有报道称，BO是在摄取了分布在东南亚的多叶灌木Sauropus androynus(SA)后发生的。然而，SA对体外培养的呼吸道驻留细胞或造血细胞的直接作用知之甚少。在这项研究中，我们研究了单核细胞系细胞、上皮细胞和内皮细胞与SA或SA提取物孵育后产生细胞因子/趋化因子的情况。用正己烷、丙酮、甲醇和水四种溶剂提取SA干粉，并将其分成多个组分。用SA溶液或SA洗脱部位与人单核细胞、人肺泡巨噬细胞、肺泡上皮细胞系(A549)和内皮细胞系(MSI)共孵育。在SA组分中，只有水提物组分能显著增加单核细胞产生的肿瘤坏死因子-α、IP-10、MIG、MDC、IL-8和VEGF。SA水提物还能抑制内皮细胞的增殖并诱导其凋亡，但对肺泡上皮细胞无明显影响。此外，水提物组分还能促进同种异体小鼠气管移植模型(活体模型)的管腔闭塞。为了进一步分配水相组分，进行了高效液相色谱分离。具有中等极性的组份可以诱导单核细胞系细胞产生大量的肿瘤坏死因子-α。这些结果表明，SA的水相部分含有可能在BO发病机制中起重要作用的物质

Recently, BO has been reported to develop following ingestion of Sauropus androgynus(SA), a leafy shrub distributed in Southeast Asia. However, little is known about direct effects of SA on airway resident cells or hematopoietic cells in vitro. In this study, we investigated the cytokines/chemokines production from monocytic lineage cells, epithelial cells and endothelial cells incubated with SA or fractions extracted from SA. Dry powder of SA was extracted with four solvents(hexan, acetone, methanol and water) and partitioned into fractions. Monocytic tumor cell lines(THP-1, RAW), human monocytes, alveolar macrophages, alveolar epithelial cell line(A549) and endothelial cell line(MSI)were incubated with SA solution or fractions eluted from SA. Among fractions from SA, only aqueous fraction induced significant increase of TNF- a, IP-10, MIG, MDC, IL-8 and VEGF production from monocytic lineage cells. The aqueous fraction of SA also suppressed the proliferation and induced apoptosis of endothelial cells but not of alveolar epithelial cells. In addition, the aqueous fraction enhanced luminal obliteration of mouse tracheal allograft model(in vivo model). For further partition of the aqueous fraction, high performance liquid chromatography was performed. A fraction with moderate polarity could induce significant TNF- a production from monocytic lineage cells. These results demonstrate that the aqueous fraction of SA contains substances that may play an important role for the pathogenesis of BO