Role of KLF15 on Energy metabolic regulation

1.肥胖发生过程中KLF 15基因的表达我们已经发现在肥胖症小鼠和高脂饮食小鼠肥胖发生过程中KLF 15基因的表达显著下调.脂肪细胞特异性KLF 15转基因小鼠的构建我们成功地构建了aP 2启动子下的脂肪细胞特异性KLF 15转基因小鼠。KLF 15的量在1号系或3号系转基因小鼠的白色脂肪组织中分别增加了1.1倍或1.3倍3。脂肪细胞特异性KLF 15转基因小鼠在正常咀嚼饲料中的代谢分析。野生型小鼠和正常咀嚼饲料喂养的KLF 15转基因小鼠之间的白色脂肪组织质量没有显著差异。而KLF 15转基因小鼠血浆脂联素浓度降低.脂肪细胞特异性KLF 15转基因小鼠高脂饮食的代谢分析高脂饮食增加了野生型小鼠的脂肪细胞质量。相反，脂肪细胞特异性KLF 15转基因小鼠维持高脂饮食不会增加体重和脂肪细胞质量。此外，口服葡萄糖耐量试验显示脂肪细胞特异性KLF 15转基因小鼠具有显著的葡萄糖耐量。脂肪细胞特异性KLF 15转基因ob/ob小鼠的代谢分析我们产生了脂肪细胞特异性KLF 15过表达的ob/ob小鼠。脂肪细胞特异性过表达KLF 15的ob/ob小鼠被发现比它们的ob/ob小鼠更小。这种体重差异至少部分归因于前一种动物的白色脂肪组织较小。6. KLF 15对SCD 1表达的影响高脂饲料喂养可使白色脂肪组织中SCD 1的表达显著上调。在脂肪细胞特异性KLF 15转基因小鼠中未观察到肥胖发展过程中SCD 1的这种上调。总之，KLF 15通过表达SCD 1/

1. KLF15 expression during the development of obesityWe have shown that the expression of the KLF15 gene is markedly down-regulated during the development of obesity in ob/ob mice and high fat-diet mice.2. Construction of adipocyte-specific KLF15 transgenic miceWe have made the 'adipocyte-specific KLF15 transgenic mice under the aP2-promoter. The amounts of KLF15 were increased by an 〜1-fold or 〜3-fold increase in white adipose tissue in line 1 or line 3 transgenic mice, respectively3. Metabolic analysis in adipocyte-specific KLF15 transgenic mice fed a normal chaw diet. The mass of white adipose tissue did not differ significantly between wild-type mice and KLF15 transgenic mice fed a normal chaw diet. However, the plasma concentration of adiponectin decreased in KLF15 transgenic mice.4. Metabolic analysis in adipocyte-specific KLF15 transgenic mice fed a high fat dietThe high fat diet increased the adipocyte mass in wild-type mice. In contrast, maintain of adipocyte-specific KLF15 transgenic mice on the high fat diet did not increase body weight and adipocyte mass. Moreover, an oral glucose tolerance test revealed marked glucose tolerance in adipocyte-specific KLF15 transgenic mice.5. Metabolic analysis in adipocyte-specific KIF15 transgenic ob/ob miceWe generated ob/ob mice with adipocyte-specific overexpression of KLF15. ob/ob mice with adipocyte-specific overexpression of KLF15 were found to smaller the their ob/ob litermates. This difference in body weight was attributable at least in part to smaller white adipose tissue in the former animals.6. Role of KLF15 on SCD1 expressionFeeding with the high fat diet resulted in a marked up-regulation of the amount of SCD1 in white adipose tissue. This up-regulation of SCD1 during the development of obesity was not observed in adipocyte-specific KLF15 transgenic mice. In conclusion, KLF15 regulates adipocyte hypertrophy via the expression of SCD1/