A role of angiotensin converting enzyme in sarcoidosis

我们研究了在单核细胞分化为多核巨细胞的过程中，ACE的表达是否增强，以及Ang-II的产生是否增强。多核巨细胞ACE的表达高于新鲜分离的单核细胞、培养的单核细胞和ConA刺激的单核细胞。但RT-PCR法不能证实ACE的mRNA水平较高，因为培养细胞上清中ACE活性较弱，培养条件无差异。荧光法检测单核细胞胞浆内ACE未检出。培养的单核细胞上清液中Ang-II水平升高。这一增量被抑制添加captril，ACE抑制剂。钙磷蛋白C、渥曼青霉素、UO 126和PP 2可抑制ACE的表达。当新鲜分离的单核细胞与M-CSF/IL-10或TGF-β/IL-10共同培养时，可诱导出CD 14 ^+ CD 16 ^+单核细胞。另一方面，当加入Ang-II时，Ang-II刺激的单核细胞的诱导被抑制，其针对单核细胞趋化蛋白-1的运动能力降低。

We investigated whether expression of ACE on monocytes is augmented in the process of differentiation into multinucleated giant cells and the production of Ang-II is enhanced. Multinucleated giant cells showed higher expression of ACE than freshly isolated monocytes, cultured monocytes and monocytes stimulated by ConA. However the higher mRNAlevel of ACE could not confirm by RTPCR method Because activity of ACE level in the supernatant of cultured cells was faint, no difference in culture condition was detected. ACE in the cytoplasm of monocytes was not detected by fluorometric assay. Ang-II levels were increased in the supernatant of cultured monocytes. This increment was inhibited by addition of captril, an inhibitor of ACE. The expression of ACE was inhibited by calphostinC, wortmannin, UO126, and PP2. The expression was also inhibited by tranilast and aloprinol.When freshly isolated monocytes were cultured in the presence of M-CSF/IL-10 or TGF-β/IL-10, CD14^+CD16^+ monocytes were induced. On the other hand, when Ang-II was added, the induction was inhibited Locomotion ability of Ang-II-stimulated monocytes was decreased against monceyte chemoattractant protein-1.