Analysis of the antitumor immunity activity of α-GalCer according to each organ against the human tumor cell line

我们用小鼠自发肝转移模型研究了α-GalCer的抗肿瘤作用机制。因此，本研究的目的是研究α-GalCer对人肿瘤细胞株的体外抗肿瘤作用。首先从人外周血或肝组织中分离淋巴细胞，并在体外加入α-GalCer。测定了对人肿瘤细胞系的细胞毒性和具有抗肿瘤活性的细胞因子的产生，并随后分析了效应细胞。此外，我们计划研究a-GalCer的抗肿瘤活性是否具有人体器官特异性。这是因为，如果α-GalCer的抗肿瘤作用具有器官特异性，则这种作用可以成为治疗癌症患者转移灶的一种方法。外周血淋巴细胞和肝淋巴细胞的抗肿瘤作用研究：从腹部手术患者的外周血和肝组织中获取外周血淋巴细胞和肝淋巴细胞，并分离外周血淋巴细胞和肝淋巴细胞。将人肿瘤细胞株和α-GalCer按不同浓度加入到每个淋巴细胞中，进行体外混合培养，用铬释放法检测细胞毒活性。但在外周血淋巴细胞和肝淋巴细胞中未能获得具有重复性的结果。因此，需要进一步的实验来确定a-GalCer的最佳浓度。干扰素-γ或白介素12的产生量不能连续评估。上述淋巴细胞的影响因素分析：对少数几个例子开始了每个淋巴细胞的效应分析。然而，我们没有得出同样具有重复性的结果。一个尽可能准备病人背景的过程和努力在未来将是最重要的。一个计划和尽可能保留患者背景的努力可能是未来最重要的。

We presented mechanism of the antitumor effect of a-GalCer using a murine spontaneous hepatic metastases model. Therefore this purpose was to investigate antitumor effect of a-GalCer against the human tumor cell line in vitro. At first lymphocytes were divided from human peripheral blood or liver tissue, and a-GalCer was added in them in vitro. Cellular cytotoxicity for the human tumor cell line and a production of cytokine having the anti-tumor activity were measured, and effectors cells were analyzed subsequently. Furthermore, we schemed to investigate whether anti-tumor activity of a-GalCer had organ specificity in a human. The reason was because a-GalCer administration could become a treatment for the metastasis lesion of the cancer patient if this antitumor effect had organ specificity.A study of the antitumor effect of peripheral blood lymphocyte and hepatic lymphocyte: From the patient who underwent abdominal operation peripheral blood and hepatic tissue were obtained, and peripheral blood lymphocytes and hepatic lymphocytes were divided. Human tumor cell line and a-GalCer were added at various kinds of concentration for each lymphocyte, and mixed culture was performed in vitro, and cytotoxic activity by the Cr release examination was measured. However, results having reproducibility could not be obtained in peripheral blood lymphocyte and hepatic lymphocyte. Therefore a further experiment was needed to establish optimal concentration of a-GalCer. Quantity of production of IFN -y or IL-12 could not be evaluated continuously.Effectors analysis of the above lymphocytes: Effectors analysis was started in each lymphocyte for a few examples. However, we did not result in an outcome having reproducibility similarly. A process and an effort to prepare as possible the background of the patient will be most important in future. A scheme and an effort to keep as possible the background of the patient may be most important in future.