Research of immunotherapy and splenectomy for liver dysfunction.

1. 造门脉高压脾大动物实验模型。我们试图建立一个动物实验模型来模拟大鼠肝损伤、门脉高压和脾肿大。采用四氯化碳处理组、芽孢杆菌处理组、牛血清白蛋白（BSA）处理组、有丝分裂原处理组、门静脉分流术（PCS）组、门静脉结扎术进行检测。经两期结扎门静脉组证实脾肿大。门静脉高压症的临床检查我们对行脾切除术的门静脉高压症患者进行血液和脾脏淋巴细胞的测定。用流式细胞仪分析淋巴细胞中活化标志物CD25、CD26、CD69、CD70的表达情况。结果表明，门静脉高压症患者术前血液中活化T淋巴细胞含量高于对照组。此外，在提取的脾脏中，早期活化T淋巴细胞的比例很高。术后血T淋巴细胞活化率短暂下降，术后第3天又升高。提示门静脉高压症患者早期活化的T淋巴细胞积聚于脾脏，成熟后流入外周血。

1. Making the animal experiment model of portal hypertension and splenomegaly.We tried to make an animal experiment model that was going to reproduce a liver damage, portal hypertension and splenomegaly with a rat experimentally. We used a carbon tetrachloride treated group, a bacillus mort treated group, a bovine serum albumin (BSA) treated group, mitogen treated group, Portocaval shunt (PCS) group, a portal vein ligation county and tested it. It was confirmed that splenomegaly was developed in the group that were ligated the portal vein with two phases.2. Clinical examination in portal hypertension casesWe adjusted lymphocyte from blood and spleen of portal hypertension patients that were performed splenectomy operation. And we analyzed the lymphocyte about expression of CD25, CD26, CD69, CD70 which was an activation marker by using the flowcyte meter. As a result, there were more activated T lymphocytes existed in preoperative blood of portal hypertension case, in comparison with control. In addition, a ratio of an early phase activated T lymphocyte was high in the extracted spleen. After operation, the ratio of the activated T lymphocyte of blood fell in transientness, but it increased again from three postoperative day. As a result, it was suggested, an early phase activated T lymphocyte was accumulated to the spleen in the portal hypertension patient, and after maturation, it flowed out into the peripheral blood.