Characterization of stress responses and its application to the lifespan regulation in model microorganisms.

为了了解微生物的应激反应策略，我对两种模式微生物--E.Coli和S.pombe进行了表征。在大肠杆菌中，我发现作为一种反应调节因子，Arca除了参与众所周知的好氧-厌氧反应外，还参与脆性细菌的合成。我还通过芯片分析鉴定了大肠杆菌转录因子ArcA、H-NS和FnR的全基因组结合位点。通过使用分裂酵母，我开始鉴定和表征与时间寿命有关的因素。在这个项目中，我确定了新的因子，Ecl1家族蛋白，当过度表达时，可以延长S.pombe的时间寿命。同时，我还分离了一些影响S庞贝寿命的突变体和克隆。基于这些研究结果，为今后的研究发展奠定了基础。

To understand the strategy of stress responses of microorganisms, I characterized two model microorganisms, E.coli and S.pombe. In E.coli, I found that a response regulator, ArcA is involved in fragella synthesis in addition to the well known aerobic-anaerobic responses. I also identified the whole genome binding sites of transcription factors, ArcA, H-NS and Fnr of E. coli by using ChIP-CHIP analysis.By using fission yeast, S pombe, I started to identify and characterize factors involved in the chronological lifespan. In this project, I identified novel factors, Ecll family proteins that increase chronological lifespan of S. pombe when overexpressed. I also isolated some mutants and clones that affect chronological lifespan of S pombe. Based on these results, I was able to establish the basis for the development of the future research.