Proteomic Analysis of Psychological Stress and Protein Higher Structures

我们假设心理应激通过改变二硫键形成和游离半胱氨酸修饰影响身体功能。我们已经证明，白蛋白的半胱氨酸残基（34^< >氨基酸）被心理应激后果氧化，这种变化引起白蛋白的高级结构改变，导致白蛋白的功能减少一半和选择性碎片化。在重度抑郁症患者的血浆样本中观察到这种特征变化。因此，我们使用LCMS程序搜索MDD患者血浆中的降解物（白蛋白片段）和/或肽穹（白蛋白相关肽）。我们在MDD患者的血浆中发现了270个仅存在的多肽，并在日本撰写了专利文件。在打开之前，里面的东西会被隐藏起来。另一方面，我们与Protosera公司和Human Metabolome Technologies公司建立了研究合作关系，前者可以通过MALDI-MS Blot-Chip系统对peptidome的数量进行简要评估，后者可以通过CE-MS对代谢组进行测量。另一份专利文件也已经写好了。细节现在不能写在这里。

We hypothesized that the psychological stress affects physical functions through alterations in disulfide bondage formation and free cysteine modifications.We have shown that the cysteine residue, 34^<th> amino acid, of Albumin is oxidated by psychological stress consequences and this change evokes Albumin higher structure alteration that leads the functional decrementation into half and alternative fragmentation of Albumin. This characteristic change is observed in the plasma samples of the patients for Major Depressive Disorder.Thus, we searched the degradome (fragments from albumin) and or peptidome (Albumin related peptides) within the plasma of MDD patients, using the LCMS procedures.We have found 270 peptides which solely existed in the plasma of MDD and wrote patent document in Japan. The contents will be hidden until it is opened.On the other hand, we made a research collaboration relationship with Protosera Company and Human Metabolome Technologies The former can evaluate the quantity of peptidome briefly by MALDI-MS Blot-Chip system, and the latter can measure the metabolome by CE-MS.The other patent document was also written already.The details cannot be written here now.