Craniofecial bone reganeration by mesenchymal stem cells of the craniofacial origin

检测人骨髓间充质干细胞（hMSCs）在颅面区移植后的动力学，转染人绿色荧光蛋白（hGFP）CDNA，研究细胞归家和分化。分别从1代到4代对转染hgfp的hMSCs进行体外对比检测。观察细胞转染效率，1代3小时和6小时转染前3天与未转染细胞相似。这些细胞用于辐照后、颅面和头颈部相关组织再生。瘢痕疙瘩临床系列也在人间充质干细胞方面进行了测试。在体内辐照后，间充质干细胞有显著的效率。此外，hMSCs被证实与纤维增生性疾病FPD有关。由于这些细胞是冷冻的，解冻后的hgfp标记是一致的，因此这些细胞可以作为储备用于紧急辐射疾病。人类间充质干细胞目前在西方国家被用于细胞库细胞。因此，在日本也应该考虑向这个方向扩展细胞。hgfp标记的hMSCs在血流量减少、神经损伤和颅骨骨缺损模型中表现出明显的恢复，并在时间和空间上进行了体内实验。因此，应该计划使用自体组织来源的干细胞进行进一步的临床前和临床试验。

Human bone marrow derived mesenchymal stem cell, hMSCs were tested for post-grafting kinetics in the craniofacial regions, human Green Fluorescent Protein (hGFP)CDNA was trasfected and then the cell-homing and differentiation were investigated. The hGFP-transfected hMSCs were comparatively tested from 1 to 4 passages in vitro. The efficiency of the cell-transfection was followed and 3-hour and 6-hour transfections in the 1 passage were similar to non-transfected cells for the first 3 days. These cells were used for post-irradiated, craniofacial-, and head and neck-related tissue regeneration. And the keloid clinical series were also tested in terms of human mesenchymal stem cells. In the post-irradiation in vivo, there was remarkable efficiency of mesenchymal stem cells. Also, hMSCs demonstrated to the relationship to the fibroproliferative disease, FPD. These cells may be used for emergency radiation diseases as stockpiles since these cells are frozen and hGFP-labeling are consistent after thawing. The human mesenchymal stem cells are now used for cell-bank cells in the Western countries. Thus, the extensions of the cells in this direction should be considered in Japan as well. hGFP-labeled hMSCs are in vivo tested in the decreased blood flows and impaired nerves demonstrated the significant recovery as well as the cranial bone-defect models temporally and spaciously. Therefore, further preclinical and clinical tests should be planned with using autologous tissue-derived stem cells.