HPV塩基配列決定による新規ハイリスクバリアントと高齢者子宮頸癌関連型の同定

目的探讨人乳头瘤病毒（HPV）型别及变异株与宫颈癌发生的关系。本研究对宫颈癌组、不典型增生组和正常对照组的宫颈细胞进行HPV序列测定，并对HPV检出的危险性进行评价。用共有引物组扩增细胞HPV序列的L1区，随后使用测序引物进行直接测序。根据对照组与癌组的比值比确定每种HPV类型和变体的风险评估。结果已识别的HPV类型数量为35种，从6型到91型，其中包括未分类的4种类型。其中33型和58型是老年人群中的高危型。HPV 16、18、31、33、35、52、58型是世界范围内流行的类型，有几种变体。其中，在浸润性癌中检测到35的16型变异体，4/4的18型变异体，1/2的31型变异体，1/2的33型变异体，1/3的35型变异体，35的52型变异体，2/5的58型变异体。虽然被归类为高危型HPV，但在浸润性癌中未检测到至少一种45、51、53、56、59、66、68、73型变异。在浸润性癌中检测到至少一种54、90、91型变异，属于低风险型。

PurposeThis research is designed for risk evaluation of human papillomavirus (HPV) types and variants for cervical carcinogenesis. In this study, HPV sequences of cervical cells of cervical cancer, dysplasia, and normal control groups were determined, and evaluated risks of detected HPVs.Material and MethodsHPV sequences of cells of cervical carcinoma group, dysplasia group, and cytologically normal group (conrtol goup) were determined. The L1 region of HPV sequences of cells were amplified with consensus primer set, and subsequently, direct sequencing was performed using sequencing primers. Risk evaluation of each HPV types and variants was determined by odds ratios of the conrol group between the carcinoma group.ResultsThe number of dentified HPV types was thirty-five, ranging from type 6 to type 91, including unclassified four types. Among them, type 33 and type 58 were the high-risk types in the elderly population.Total of 85 genotypes of HPV were identified in this study. HPV types 16, 18, 31, 33, 35, 52, 58, world-wide popular types, have several variants. Among them, three out of five variants of type 16, all four variants of type 18, one out of two variants of type 31, one out of two variants of type 33, one out of three variants of type 35, three out of five variants of type 52, all two variants of type 58 were detected in the invasive carcinoma. Although classified into the high-risk HPV, at least one variants of types 45, 51, 53, 56, 59, 66, 68, 73 were not detected in the invasive carcinoma. Reversely, classified into the low-riks HPV, at least one variants of types 54, 90, 91 were detected in the invasive carcinoma.