2G allile of MMP-1 gene polymorphism increases risk of oral cancer

为了建立一种有效的骨再生方法，以弥补牙槽嵴的缺损，我们尝试将自体培养的骨膜细胞与骨移植物联合应用。2006年，在生物洁净室中，由专职技术人员和盐委员会进行了系统的骨膜细胞培养，随后进行了自体培养骨膜在牙槽骨增量中的临床试验。(1)系统性骨膜细胞培养的条件：生物洁净室的建设和培养专职技术人员的培训;伦理委员会和软组织委员会的建立（2006年）。(2)临床前试验结果：在实际培养系统中对培养程序、血清和培养基以及其他试剂进行评估。检查细胞生长和形态，细菌或病毒或支原体感染，以及牛血清残留水平。在试验培养物中未观察到异常结果 ...更多信息 .在培养的第一阶段，通过巢式PCR分析检测到微生物的污染，但在培养结束时消失。牛血清残留量在免疫荧光法和斑点杂交法的检测限以下。未估计肿瘤形态学，如结节生长或增生。ALP活性在整个培养过程中得以维持，表明成骨细胞表型一直维持到培养结束。(3)在本项目期间经历了7例自体骨膜细胞移植。3例牙槽静脉植骨，4例上颌窦底提升术后辅以自体骨膜细胞。7例病例中有2例进行了假体治疗，未报告异常愈合过程，也未报告移植物感染。移植区的CT图像显示骨形成良好，移植物吸收较少。少

Tb establish a effective procedure of bone regeneration compensating deformed or low alveolar ridge for dental implantology, we attempted the administration of autologous cultured periosteal cell with the bone graft. Conditoning of systemic periosteal cell culture, in tearms of a biological clean room with full-time tecnitian and committee for the salty, in 2006 was followed by clinical test of the autologous cultured periosteum in augmentation of alveolar bone.(1) Conditoning of systemic periosteal cell culture: Construction of the biological clean room and training of full-time tecnitian for the culture; establishment of ethical and softy committees (2006).(2) Pre-clinical test sultures: estimation of the culture procedure, serum and mediums, and other reagents were performed in the actual culture system. Growth and morphology of cells, bacterial or virus or micoplasm infections, and remnant level of the bobine serum were examined. No abnormal finding was observed in the test culture … More . Growth arrest was observed on sixth week after the onset of cram At the first periods of the culture, contamination of micoplasms were detected by nested-PCR analysis, but were disappeared at the end of the culture. Bovine serum remnant was under the detection limit by the immunofluorosent or dot-blot methods. No oncologic morphology, such as nodal growth or atypism, was estimated. ALP activity was maintained through the culture process, suggesting osteoblastic phenotype was maintained until the end of the culture.(3) seven cases of autologous periosteal cell graft were experienced within the period of the project. Three of alveolar venia bone grafts, four cases of sinus floor elevations were supplemented by autologous periosteal cells. Two of the seven cases have been performed with prosthetic treatment No abnormal healing process, as well as no infection of the grafts was reported. CT image of the grafted area suggested well developed bone formation with less resorption of the graft. Less