Pulification, structure and characterization of anti-tumor compounds from marine organisms and analysis of their mechanisms

(1)海洋生物的纯化和抗癌化合物的研究。海洋生物活性物质的纯化、结构。海洋生物抗肿瘤化合物的分离及其对口腔鳞状细胞癌细胞的细胞毒性评价。从日本近海收集海洋生物并冷冻干燥。用乙醇-甲醇（4：1）均质，用高效液相色谱（HPLC）分别分离正己烷层、C_2H_5OCH_3CO层和水层。并对其进行了纯化和结构鉴定。从海洋生物中提取的生物活性物质，通过对生物活性物质分枝结构的改进，获得了具有抗肿瘤活性的药物。（2）对鳞状细胞癌细胞的抗肿瘤活性分析。采用无血清生物测定法，研究了海洋生物中提取的生物活性物质对人癌细胞的抗肿瘤作用。对HAS法尼基化的抑制分析。(3)海洋生物对接种人癌细胞的抗肿瘤作用分析用Balb-c/nu/nu裸鼠接种人口腔鳞癌细胞和A431细胞，观察海洋生物对人口腔鳞癌细胞的体内抗肿瘤作用。随后，将部分化合物与载体一起腹腔注射，以肿瘤体积为指标评价其抗肿瘤作用。

(1) Purification of marine organisms and search of anti-cancer compounds from marine organisms.Purification, structure of active biological substances from marine organisms.Isolation of anti-neoplastic compounds from marine organisms and evaluation of their cytotoxity against oral squamous cell carcinoma cells. Marine organisms from Japanese adjacent Sea were collected and freeze-dried. They were homogenized in EtOH-MeOH(4:1).They were distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer., proceedingly, were investigated their activity by bin assay and were purificated and decided their structure. Nobel agents were derived from active biological substances improving the structure of their branches(2) Analysis of antitumor activity on squamous cell carcinoma cellsActive biological substances pulified from marine organisms were examined on the antitumor effect of human cancer cells with serum-free bio assay.Analysis of anti-angiogenetic effect of marine organisms.Analysis of the effect of tumor cell attachment, invasion and metastasis.Analysis of inhibition against Has farnesylation.(3) Analysis of the anti-tumor effect of marine organisms on inoculated human cancer cell Anti-tumor activity against human cancer cells (oral squamous cell carcinoma and A431) were investigated in vivo.OSCC cells were inoculated on Balb-c/nu/nu nude mices. Proceedingly, some compounds were injected with vehicle intraperitoneally.Anti-tumor effect of these compounds were consequently evaluated their tumor volume.