Effects of chemotherapy and angiogenesis inhibitor on invasion and metastasis of oral squamous cell carcinoma

利用原位种植模型，其中的口腔癌的侵袭和转移可以重现，我们研究了抗癌药物对侵袭和转移的抑制作用。将高度侵袭性和转移性的人口腔鳞状细胞癌细胞系OSC-19植入裸鼠的口腔底，并在植入后7或14天向小鼠施用顺铂或培洛霉素。研究了每种抗癌药物和不同给药时间对癌症侵袭和转移的影响。肿瘤大小和增殖细胞核抗原阳性细胞的比例显着减少。在对照组中，肿瘤显示出4C级的侵袭模式，而在用抗癌药物治疗的组中，在77.3%的小鼠中观察到3级，观察到对肿瘤侵袭的抑制作用。植入后第7天，接受顺铂或培洛霉素治疗的组颈部淋巴结转移率显着降低。转移性淋巴结中的肿瘤分期进展也受到抑制。化疗不仅能使肿瘤缩小，而且能抑制肿瘤的侵袭和转移。考虑到这些影响，使用抗癌药物可能在临床上非常有用。

Using an orthotopic implantation model in which the invasion and metastasis of oral cancer can be reproduced, we investigated the inhibitory effects of anticancer agents on invasion and metastasis. A highly invasive and metastatic human oral squamous cell carcinoma cell line, OSC-19, was implanted into the oral floor of nude mice, and cisplatin or peplomycin was administered to the mice 7 or 14 days after the implantation. The effects of each anticancer drug and different administration timings on cancer invasion and metastasis were investigated. Tumor size and the ratio of proliferating cell nuclear antigen positive cells were significantly reduced. In the control group, the tumors showed grade 4C of mode of invasion, while in the groups treated with anticancer drugs, grade 3 was observed in 77.3% of the mice, with an inhibitory effect on tumor invasion being observed. The rate of metastasis in the cervical lymph node was significantly decreased in the groups treated with the cisplatin or peplomycin on day 7 after the implantation. The tumor stage progression in the metastatic lymph nodes was also inhibited. Chemotherapy is effective not only for tumor diminution, but also for inhibiting invasion and metastasis. The use of anticancer drugs considering these effects may be clinically very useful.