A genomic approach to the mechanism of meiotic recombination in Neurospora

在高等生物生命周期的还原分裂过程中，重组使DNA序列在同源染色体之间发生改组。与突变一起，它也是进化中的一个关键过程。对重组所涉及的分子过程的了解在很大程度上是基于酵母的，酵母不能容忍严重的序列错配，限制了对正常重组事件的分析的分辨率。我们已经证明，像其他不易处理的多细胞真核生物一样，脉孢子虫对序列错配具有耐受性，允许对单个重组事件进行高分辨率分析。这个项目将建立在我们在理解重组如何发生方面已经取得的根本性进展的基础上。

Recombination shuffles DNA sequences between homologous chromosomes during the reduction division in the life cycle of higher organisms. Along with mutation, it is a key process in evolution. Understanding of the molecular processes involved in recombination is largely based on yeast, which is intolerant of significant levels of sequence mismatch, limiting the resolution of analyses of normal recombination events. We have shown that Neurospora, like other less tractable multicellular eukaryotes, is tolerant of sequence mismatch, allowing high resolution analysis of individual recombination events. This project will build on fundamental advances we have already made in understanding how recombination occurs.