Development of next generation nano-biomaterials by cell surface engineering

我们尝试了一种方法来构建二维和三维组织使用配体固定的聚合物链作为人工细胞外基质。这种基于细胞聚集体工程的技术可以有效地对具有复杂形状的人工材料进行细胞涂覆，以改善生物相容性并促进组织重建。接下来，我们提出了一种新的方法，用于增强配体-受体结合和修饰细胞表面作为人工材料。因此，我们制备了一种由配体、光反应基团、生物素和二硫键组成的多功能聚合物（聚合物纳米工具）。我们发现，细胞修饰的生物素携带的纳米工具特异性地粘附到亲和素固定的板和细胞聚集体的形成介导的加入亲和素。

We tried an approach to construct 2- and 3-dimensional tissues using a ligand-immobilized polymer chain as an artificial extracellular matrix. This technique based on the cell-agglomerate engineering allowed the efficient cellular coating of artificial materials with complicated shapes for improvement of biocompatibility and facilitation of tissue reconstruction. Next, we proposed a novel method for enhancing ligand-receptor binding and modifying cell surface as artificial material. So, we prepared a multi-functional polymer (polymer nano-tool) consisted of ligand, photo-reactive group, biotin and disulfide. We found that cells modified with biotin-carrying nano-tools specifically adhered to an avidin-immobilized plate and the formation of cell agglomerates was mediated by adding avidin.