Development of a new culture process to prevent an oncogenic transformation of the stem cells

本研究的目的是开发一种新的培养过程，以防止肝干细胞的致癌转化，使其安全应用于再生医学。首先，我们建立了三种干细胞致癌转化的估计方法。其次，我们发现多孔羟基磷灰石培养载体适合干细胞的大量培养，其表面的孔隙大小和凹凸度对阻止干细胞的致癌转化和增强干细胞的肝脏活性具有重要作用。第三，我们利用本研究最初开发的杂交脂质体，成功地选择性消除了培养干细胞中的转化细胞。

The aim of this research is to develop a new culture process to prevent an oncogenic transformation of hepatic stem cells for their safety application to regenerative medicine. First, we established three estimation methods for the oncogenic transformation of the stem cells. Second, we found that the porous hydroxyapatite culture carrier was suitable for mass culture of the stem cells, and the pore size and raggedness of the surface were important to prevent the oncogenic transformation and enhance the hepatic activity of the stem cells. Third, we succeeded in the selective elimination of transformed cells in the cultivated stem cells by using hybrid liposomes, which originally developed in our research.