Molecular mechanisms for dissolution of water-insoluble minerals containing phosphates by mycorrhizal fungi

外生菌根（ECM）真菌可溶解含磷的水不溶性矿物质，使磷酸盐以可溶性形式被寄主树吸收。强螯合剂，如草酸盐、柠檬酸盐和苹果酸盐，从ECM中释放出来，在通过络合溶解矿物质中起关键作用。在这项研究中，我们试图克隆一个编码草酸产生酶细胞色素c依赖乙醛酸脱氢酶同源基因的cDNA。我们从古Fomitopsis palustris中分离到了编码细胞色素c依赖性乙醛酸脱氢酶（FpGLOXDH）的cDNA。我们发现FpGLOXDH同源基因（jgi|Lacbil|296363|er2 lbscolog 01720, Laccaria bicolor）存在于Laccaria bicolor的基因组中。而在双色l中未检测到FpGLOXDH的活性。这些有机酸的生物合成机制有待进一步研究。

Ectomycorrhizal (ECM) fungi solubilize water-insoluble minerals containing phosphorus, making phosphate available in soluble form to be taken up by the host tree. Strong chelators, such as oxalate, citrate, and malate, released from ECM play a pivotal role in the dissolution of minerals through complexolysis. In this study, we attempted to clone a cDNA encoding a homologue of the oxalate-producing enzyme cytochrome c dependent glyoxylate dehydrogenase from Laccaria bicolor. We isolated cDNA encoding the cytochrome c dependent glyoxylate dehydrogenase (FpGLOXDH) from Fomitopsis palustris. We found that the homologue of FpGLOXDH (jgi|Lacbil|296363|er2 Lbscfoolog 01720, Laccaria bicolor) was present in the genome of L.bicolor. However, activity of FpGLOXDH was not detected in L.bicolor. Further research is needed to elucidate the mechanism underlying the biosynthesis of these organic acids.