Toxicokinetics of organophosphorus insecticide-Development and application of the analytical method for the metabolites by CE-MS-

建立了用毛细管电泳质谱(CE/MS)测定有机磷农药代谢物二烷基磷酸盐(DAPs)的方法，并对有机磷农药中毒患者的血清和尿液进行了检测。用CE-MS检测7个DAP。在29例有机磷农药中毒病例中，用该方法检测到了预期的DAP。O、S-DMTP也被检测到。通过对DAPs的测定，可以区分摄入有机磷杀虫剂的磷酸盐类型和单一或混合配方。血清DMP浓度与中毒症状程度有关。

We developed a determination method for the dialkylphosphates (DAPs), metabolites of organophosphate insecticides, using capillary electrophoresis/mass spectrometer (CE/MS) and applied this method to serum and urine collected from poisoned patients with organophosphate insecticide. The 7 DAPs were detected by CE-MS. In all of 29 caces of organophosphate insecticide poisoning, the expected DAPs were detected using this method. O,S-DMTP was also detected. The phosphate type and single or mix formulation of ingested organophosphate insecticide could be distinguished by determination of DAPs. There was a relation between DMP concentration in the serum and the degree of toxic symptoms.