The role of ASIC for pathophysiology of migraine

在这项研究中，我们探讨了酸感离子通道（ASIC）和瞬时受体电位香草蛋白亚家族成员1 （TRPV1）在偏头痛病理生理中的作用。除了血管功能障碍外，三叉神经节支配的硬脑膜的神经源性炎症也被认为与偏头痛的病理生理有关。我们在这个项目中证明了TRPV1受体在硬脑膜和三叉神经节中的存在。此外，我们的数据表明，硬脑膜中TRPV1受体的伤害性刺激导致ERK磷酸化，这是对三叉神经节外周TRPV1受体在体内的有害刺激的响应。我们还在体外观察了ERK的磷酸化。由于发现TRPV1刺激后这种磷酸化迅速发生，我们推测ERK磷酸化可以在三叉神经节水平上作为伤害感觉的敏感标志物。我们还观察了大鼠复张静脉的发育模式以及复张静脉与硬脑膜TRPV1受体的关系。许多硬脑膜TRPV1受体免疫反应神经纤维在复张静脉上被观察到，这些发现表明来自复张静脉的热量可能影响到TRPV1受体的阈值

In this study, we investigated the role of acid sensing ion channel (ASIC) and the transient receptor potential vanilloid subfamily member 1 (TRPV1) for pathophysiology of migraine. In addition to vascular dysfunction, the neurogenic inflammation of the dura mater innervated by the trigeminal ganglion is considered to be related to migraine pathophysiology. We demonstrated the existence of the TRPV1 receptor in the dura mater and the trigeminal ganglion in this project. Furthermore, our data showed that the nociceptive stimulation of TRPV1 receptors in the dura mater caused the phosphorylation of ERK, which is in response to the noxious stimulation of peripheral TRPV1 receptors in the trigeminal ganglion in vivo. We also observed the phosphorylation of ERK in vitro. As such phosphorylation was found to occur rapidly after TRPV1 stimulation, we surmised that ERK phosphorylation can serve as sensitive marker of nociception at the level of the trigeminal ganglion. We also observed the developmental pattern of the diploic veins and the relationship between the diploic veins and the dural TRPV1 receptor. Many dural TRPV1 receptor immunoreactive nerve fibers were observed over the diploic veins and these finding indicated the possibility that heat from the diploic veins may affect to the threshold of the TRPV1 receptor