Regeneration of Enamel using Cultured Ameloblast

牙齿缺陷的基本治疗方法是再生那些因牙齿缺陷而失去的牙齿。然而，为了将这种牙齿器官再生应用于医疗，牙齿萌出、大小和形态的控制、再生周期等问题都被留在了陷阱中。因此，这并不被认为是万无一失的治疗方法。因此，目前常用的修复方法有桥接、局部义齿、种植体等。另一方面，创伤或先天性/获得性牙齿缺陷是通过人工材料修复的，如金属、陶瓷和树脂。然而，与天然牙齿相比，这些人造材料在生物相容性、美学、功能性方面确实存在各种问题。这项研究描述了再生疗法是如何应用于临床的。作为第一步，我们研究了应用组织工程技术的牙齿再生，特别是釉质再生和牙齿修复材料。最近，利用培养的牙蕾细胞进行釉质再生的研究已有报道。然而，研究发现，釉质再生不仅需要很长的时间才能完成组织再生，而且会使再生的组织过早和小型化。此外，有研究表明，细胞外基质包括I型胶原和纤维连接蛋白对牙上皮细胞的生长和分化有促进作用。这项研究考察了培养的牙源性细胞分层如何影响细胞的生长和分化以及组织再生的能力。在此基础上，对细胞培养和组织再生方法进行定性和定量的论证。

The fundamental treatment for a tooth defect is to regenerate those teeth that have been lost as a result of the defect. However, in order to apply this tooth organ regeneration to medical treatment, issues such as tooth eruption, control of size and morphology, regeneration period, are left as pitfalls. Thus, this is not regarded as a foolproof treatment. Therefore, at present prosthesis treatments such as bridge, partial denture and dental implant are commonly performed. On the other hand, trauma or congenital/acquired tooth defect is restored by artificial materials, such as metal, ceramic and resin. However, it is true that these artificial materials have various issues in terms of biocompatibility, esthetics, functionality, compared to natural teeth. This study delineates how regenerative therapy is applied to clinical application. As a first step, we examined tooth regeneration which applied tissue engineering technique, especially enamel regeneration and tooth prosthesis material. Recently, enamel regeneration, which utilizes culture tooth bud cell, has been reported. However, it is found that not only does enamel regeneration take a long period to complete tissue regeneration but also turn regenerated tissue premature and small. In addition, it has been reported that the extracellular matrix including collagen type 1 and fibronectin promoted the growth and differentiation of cultured dental epithelial cell. This research examines how cultured odintogenic cell stratification will affect the ability of cell growth and differentiation and tissue regeneration. Then, it intends to prove cell culture and tissue regeneration method quantitatively and qualitatively.