Development of a screening method for drugs in formalin-fixed tissue

本研究旨在建立催眠药、抗抑郁药、抗精神病药等38种中枢神经系统药物的筛选方法。将药物标准品加标至猪肝的甲醇匀浆中，然后按以下顺序进行净化：i）通过冷却进行脂肪沉淀，ii）己烷-乙腈分配，iii）在碱性条件下进行液-液萃取。制备的样品通过液相色谱串联质谱法测定。除7-氨基苯二氮卓类药物（3种）外，其他靶向药物的回收率和重复性（CV %）分别大于40%和15%。该方法可用于实体组织中的药物筛选。

The aim of this study is to develop a screening method for 38 central nervous system drugs such as hypnotics, antidepressants, antipsychotics. The drug standards were spiked to methanol homogenate of porcine liver, then clean-up was performed as following order : i) fat precipitation by cooling, ii) hexane-acetonitrile partition, iii) liquid-liquid extraction under basic condition. The sample prepared was determined by liquid chromatography tandem mass spectrometry. The recovery and repeatability (CV%) of the targeted drugs except for 7-aminobenzodiazepines (3 drugs) were more than 40% and within 15%, respectively. The method would be useful for drug screening in solid tissues.