Global gene expression in periodontopathogeic bacterium biofilm

采用DNA芯片技术对成人牙周炎主要病原之一的牙龈卟啉单胞菌生物膜细胞与浮游细胞的基因表达进行了分析。上调的基因有营养摄取和信号分子分泌基因、能量代谢基因、双组分系统基因等。下调基因有热休克蛋白基因、氧化应激转录基因、磷酸酶基因等。本研究表明，牙龈假单胞菌生物膜细胞与菌斑细胞之间存在基因表达差异。此外，这表明ABC转运体与牙龈假单胞菌生物膜的形成有关。

Gene expression analysis between biofilm cells and planktonic cells of Porphyromonas gingivalis which is one of the major aetiological agents in advance adult periodontitis was done with DNA microarray. Upregulated genes were gene for uptake of nutrient and secretion of signal molecules, gene for energy metabolism, gene for two component system and so on. Downregulated genes were gene for heat shock protein, gene for transcription under oxidative stress, phosphatase and so on. In this study, it was shown that difference of gene expression occurred between biofilm cells and plaktonic cells of P. gingivalis. Besides, it implies that that ABC trasporters are associated to formation of P. gingivalis biofilm.