Development of Bioassay System with Microwell Slide Towards High throughput Single Cell Analysis

由于大多数用于细胞分析的实验室生物测定都是为了测量对细胞群的平均影响而设计的，因此很难以高通量的方式研究单个细胞的特征，例如患者的肿瘤细胞样本。最近，一种新型的微孔载玻片已经开发出来，它可以将数千个单细胞定位到一个精确的位置，培养数周，并以高通量单独处理。在本项目中，我们提出了一种由集成在微孔板上的有源微流控装置组成的生物测定系统，以改善对单细胞的控制，并为单细胞提供了新的应用。

Since most laboratory bioassays used for cell analysis are designed to measure average effects on cell population, it is difficult to study characteristics of individual cells such as patient's tumor cell samples in a high throughput manner. Recently, a novel microwell slide that allows several thousands of single cells to be addressed to an exact position, cultured for weeks, and treated separately in high throughput has been developed. In this project we have proposed a bioassay system consisting of an active microfluidic device integrated on the microwell plate for improved control of, and new applications for the single cells.