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Molecular Biological Analysis on Glomerular Function by Multi-Photon Confocal Laser Microscopy

Molecular Biological Analysis on Glomerular Function by Multi-Photon Confocal Laser Microscopy
多光子共焦激光显微镜对肾小球功能的分子生物学分析
批准号:
20300163
负责人:
NAKAMOTO Hiroshi
金额:
$12.56万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010

项目摘要

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中文摘要
翻译
我们能够在一只大鼠身上观察到肾小球滤过。糖尿病早期即可见较大颗粒渗漏,其相对分子质量为70kDAL。造成渗漏的原因是发生过滤的缝隙膜结构发生了变化。这是由于糖尿病导致裂隙膜组分蛋白Podocin和CD2AP的排列异质性损伤所致。
英文摘要
We were able to visualize glomerular filtration in a rat. There was leakage of larger particles with the molecular weight of 70k Dal, which was found progressive even at the early stage of diabetes. The reason of this leakage was attributed to the structural changes of the slit membrane where filtration takes place. It is because alignment of podocin and CD2AP, component proteins of the slit membrane was heterogeneously damaged due to diabetes.
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会议论文
Visualisation of nephroprotective function of C-peptide in an early stage diabetic rat
C肽对早期糖尿病大鼠肾保护功能的可视化
DOI: --
发表时间: 2010
期刊: 生体医工学
影响因子: --
作者: [Yokoyama N, Ohsawa H, Waguri S, Machida S, Yoshikawa M, Fujimoto T, Takatani S., Hiroshi Nakamoto]
通讯作者: Hiroshi Nakamoto
In-vivo Visualisation of glomerular filtration by two-photon microscopy in a rat
通过双光子显微镜观察大鼠肾小球滤过的体内可视化
DOI: --
发表时间: 2009
期刊: IFMBE Proceedings 25
影响因子: --
作者: [Asoda S, Arita T, Koshitomae H, Takakuda K., 荒井謙, Zhang GX, Hiroshi Nakamoto]
通讯作者: Hiroshi Nakamoto
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [仲本博]
通讯作者: 仲本博
In-vivo Visualisation Study of Renal Microcirculation in a Rat
大鼠肾脏微循环的体内可视化研究
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [仲本博, 矢田豊隆, 小笠原康夫, 藤原宏志, Takakuda K, 仲本博, Hiroshi Nakamoto]
通讯作者: Hiroshi Nakamoto
共 11 条
    Biomolecular analysis on disturbance of glomerular filtration at the early stage of diabetes
    • 批准号:
      23650278
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.33万
    • 财政年份:
      2011
    • 负责人:
      NAKAMOTO Hiroshi
    • 依托单位:
    Analysis on regulation of renal microcirculation with an in-vivo visualisation system
    • 批准号:
      17300164
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $9.34万
    • 财政年份:
      2005
    • 负责人:
      NAKAMOTO Hiroshi
    • 依托单位:
    Construction of an analysis system on acute renal failure pathogenesis by direct measurement of renal microcirculation haemodynamics
    • 批准号:
      14380418
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $9.09万
    • 财政年份:
      2002
    • 负责人:
      NAKAMOTO Hiroshi
    • 依托单位:
    Analysis on renal glomerular capillary haemodynamics with a high speed CCD intra-vital videomicroscope
    • 批准号:
      12558115
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $7.36万
    • 财政年份:
      2000
    • 负责人:
      NAKAMOTO Hiroshi
    • 依托单位:
    海外基金