Mechanism of abnormal protein aggregation and the study of the repair enzyme.

从年龄相关疾病，如白内障，年龄相关性黄斑变性和太阳弹性变性的蛋白质异常聚集已被观察到。在这项研究中，我们检查了老年人白内障眼晶状体和紫外线损伤皮肤中生物学上不常见的d -氨基酸。在紫外照射和氧化应激的作用下，蛋白质中D-氨基酸的形成加快。我们提出d -氨基酸可能是蛋白质异常聚集的触发因素之一，可以诱导相应蛋白质的部分展开，导致患病状态。我们展示了一种用LC-MS分析蛋白质中单个Asp残基异构化的新方法。这使得精确、快速地分析蛋白质中Asp残基的异构体成为可能。

Abnormal aggregation in protein from age-related diseases such as cataracts, age-related macular degeneration and solar elastosis has been observed. In this study, we examined the biologically uncommon D-amino acids in the cataractous eye lens and UV-damaged skin of elderly individuals. The formation of the D- amino acids in the protein was accelerated by UV-irradiation and oxidative stress. We proposed that D-amino acids may be one of the triggers for abnormal aggregation of proteins and can induce the partial unfolding of the corresponding proteins, leading to a diseased state. We demonstrated a new method for the analysis of isomerization of individual Asp residues in proteins using LC-MS. This makes it possible to analyze isomers of Asp residues in proteins precisely and quickly.