Studies on regulation of matrix ion dynamics and energy metabolism by mitochondria NCX

本研究的目的是定量阐明线粒体的生理功能。采用钙敏感荧光染料研究心肌细胞线粒体和细胞质钙动力学。在此基础上，结合前人的研究成果，建立了一个能较好地再现线粒体Ca、Na、H变化的线粒体数学模型。将该模型与氧化磷酸化和TCA循环等代谢模型相结合，建立了一个完整的线粒体模型。这表明，钙和磷酸盐依赖的调节过程是重要的，在保持能量代谢平衡。

The aim of this study was quantitative elucidation of physiological function of mitochondria. Mitochondrial and cytoplasmic Ca dynamics were studied by using Ca-sensitive fluorescent dyes in cardiac myocytes. Based on these data and previously published data, a mathematical model of mitochondria which can well reproduce mitochondrial Ca, Na and H changes was newly developed. A comprehensive mitochondria model was accomplished by integrating the model with the metabolic model including oxidative phosphorylation and TCA cycle. It was suggested that Ca- and phosphate-dependent regulatory processes are important in keeping the balance of energy metabolites.