Study on structure and function of mitochondria Na-Ca exchange(NCLX)

线粒体Na-Ca交换(NCLX)结构与功能研究

• 批准号: 23390042
• 负责人: MATSUOKA Satoshi
• 金额: $ 12.65万
• 依托单位: University of Fukui (2013)Kyoto University (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390042/
• 关键词: トランスポーター; ミトコンドリア; カルシウム; 心筋細胞; リンパ球; シミュレーション; Na-Ca交換; 自動能

In B lymphocyte cell lines (A20 and DT 40), it was demonstrated that mitochondrial Na/Ca exchanger (NCLX) is associated with mitochondrial Ca extrusion, modulation of endoplasmic reticulum Ca content, endoplasmic reticulum Ca release upon BCR-stimulation, store-operated Ca entry and chemotaxis. In cardiomyocyte cell line (HL-1), it was also demonstrated NCLX extrudes mitochondrial Ca and modulates sarcoplasmic reticulum Ca content. Additionally, it was found that NCLX modulates spontaneous beating frequency. These results were well reproduced by mathematical models of B lymphocyte, HL-1 cell and sinoatrial node cell. Taken together, it was suggested that NCLX act as a Ca provider from mitochondria to sarco- and endo-plasmic reticulum.

在B淋巴细胞系（A20和DT 40）中，已证实线粒体Na/Ca交换器（NCLX）与线粒体Ca外排、调节内质网Ca含量、BCR刺激时内质网Ca释放、钙库操纵的Ca内流和趋化性有关。在心肌细胞株HL-1中，NCLX也被证实挤压线粒体Ca并调节肌浆网Ca含量。此外，发现NCLX调制自发搏动频率。这些结果与B淋巴细胞、HL-1细胞和窦房结细胞的数学模型很好地吻合。两者合计，这表明，NCLX作为一个钙供应商从线粒体到肌质网和内质网。

项目成果

Mechanism for NCLX reduction-mediated slowing of cardiomyocyte beating rate

NCLX 减少介导的心肌细胞跳动率减慢的机制

• 发表时间: 2012
• 作者: Takeuchi A;Kim B;Matsuoka S
• 通讯作者: Matsuoka S

新訂・生理学実習書 : 心室筋細胞興奮収縮連関のシミュレーション実習

新修订生理学训练书：心室肌细胞兴奋-收缩耦合模拟训练

• 发表时间: 2013
• 作者: 竹内綾子;松岡達
• 通讯作者: 松岡達

Simulation analyses of the role of NCLX, a mitochondrial Na^+-Ca^<2+> exchanger, on the regulation of automaticity in sinoatrial node cells

线粒体Na^-Ca^2交换剂NCLX对窦房结细胞自律性调节作用的模拟分析

• 发表时间: 2013
• 作者: Takeuchi A;Matsuoka S
• 通讯作者: Matsuoka S

Mitochondria Na(+)-Ca (2+) exchange in cardiomyocytes and lymphocytes.

• DOI: 10.1007/978-1-4614-4756-6_16
• 发表时间: 2013
• 期刊: Advances in experimental medicine and biology
• 作者: Bongju Kim;A. Takeuchi;Orie Koga;M. Hikida;S. Matsuoka

ミトコンドリア-(筋)小胞体Ca^<2+>クロストークと細胞機能連関

线粒体-(肌)内质网Ca^<2+>串扰与细胞功能联系

• 发表时间: 2014
• 作者: 竹内 綾子;金 鳳柱;松岡 達
• 通讯作者: 松岡 達