IDENTIFICATION OF MOLECULAR MARKERS FOR ADVANCED PROSTATE CANCER AND CLARIFICATION OF MOLECULAR MECHANISM DURING PROGRESSION OF PROSTATE CANCER

使用SELDI-TOF MS，我们对去势抵抗前列腺癌患者的血清进行了蛋白质质量分析，发现了载脂蛋白C-I(ApoC-I)，它的水平随着疾病的进展而增加。MicroRNAs(MiRNAs)是一种调节基因表达的非编码小RNA。我们研究了这些miRNAs(miR-145、miR-1和miR-133a)在前列腺癌细胞中的功能意义，并确定了新的分子靶点--嘌呤核苷磷酸化酶(PNP)和筋膜蛋白同源物1(FSCN1)。我们发现，在大鼠和小鼠前列腺癌模型中，由复制能力强的和不可传播型的重组仙台病毒(RSeV)激活的树突状细胞(DC)可以抑制肺转移。

Using SELDI-TOF MS, we performed protein mass profiling of the sera from the patients with castration-resistant prostate cancer and identified apolipoprotein C-I (ApoC-I), which level increased with disease progression. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression. We examined the functional significance of such miRNAs (miR-145, miR-1 and miR-133a) in prostate cancer cells and identified the novel molecular targets, purine nucleoside phosphorylase (PNP) and Fascin homolog 1 (FSCN1). We showed that lung metastases were suppressed by the use of dendritic cells (DCs) activated by replication-competent, as well as nontransmissible-type, recombinant Sendai viruses (rSeV), in both rat and mouse prostate cancer models.