Analysis of Intra-cellular signaling pathway and gene network in acute lung injury as for therapeutic strategy.

急性呼吸窘迫综合征（ARDS）后肺纤维化的发病率和死亡率较高。因此，深入研究肺纤维化的发生机制和致纤维化的关键分子，将有助于ARDS的治疗。用皮下植入的渗透泵持续给予博莱霉素，造成大鼠肺纤维化。在博莱霉素给药后8天，当肺纤维化发生时，向大鼠体内给予表达角质细胞生长因子（KGF）的腺病毒载体。治疗组肺纤维化、氧合损害和死亡率减轻。施用博莱霉素后肺中增加的转化生长因子（TGF）-β在施用KGF载体后减少，这表明TNF-β对肺纤维化的重要性。提示上皮生长因子对急性呼吸窘迫综合征具有肺保护作用。

Lung fibrosis following ARDS has high morbidity and mortality. We thought that investigation on the mechanism on lung fibrosis and key molecules causing fibrosis should be of benefit to ARDS treatments. Giving bleomycin continuously using an osmotic pump implanted subcutaneously, we crated pulmonary fibrosis on rats. Eight days after the administration of bleomycin when lung fibrosis occurs, rats were given keratinocyte growth factor (KGF) expressing adenovirus vector intratracheally. The treatment group expressed attenuated pulmonary fibrosis, oxygenation impairment, and mortality. Transforming growth factor (TGF)-beta that increased in lungs after the administration of bleomycin was reduced with KGF vector administration, which suggested the importance of TNF-beta on pulmonary fibrosis. These observations suggested the lung protective effects of epithelial growth factor on ARDS.