Development of novel antiangiogenic therapy based on tumor microenviroenment

为了开发更能反映肿瘤微环境的新型抗血管生成药物，本研究比较了肿瘤内皮细胞(TEC)和正常内皮细胞(NEC)的基因表达，通过DNA芯片分析确定了TEC特异的基因，提取了这些基因，并通过siRNA法分析了这些分子在TEC中的功能，敲除了一些基因，抑制了TEC的存活和迁移。此外，对几种TEC标志物的抑制显示出体内肿瘤生长的抑制。这些结果表明，它使我们能够开发出针对TEC的新型抗血管生成药物。

To develope novel anti-angiogenic therapy reflecting on tumor microenvironment more, we compared gene expression between tumor endothelial cell (TEC) and normal endothelial cell (NEC) and identified TEC specific genes by DNA microarray analysis.We picked up these genes and analyzed the function of these molecules in TECs by knock down using siRNA.Knock down of some genes inhibited TEC survival and some inhibited cell migration on TEC. Furthermore, inhibition of several TEC markers showed tumor growth suppression in vivo. These results suggested that it enabled us to develope novel anti-angiogenic therapy targeting TEC specifically.