Chemical synethesis of marine cyclic peptides for elucidation of their stereostrutures and biological activities.

为了确定帕劳酰胺的绝对立体化学性质，我们合成了四种可能的帕劳酰胺的C37和C38非对映体。在4个合成非对映体中，1与天然帕劳酰胺的核磁共振波谱完全一致。这就建立了完整的立体结构。对金耳内酯进行了SAR研究，确定金耳内酯的靶生物分子为线粒体内的禁止素。对酰胺的合成进行了研究。Miuraenamide诱导肌动蛋白聚合并稳定肌动蛋白丝。此外，还进行了相关化合物的分离、结构测定和合成研究。

To determine the absolute stereochemistry of palau'amide we synthesized four possible diasteremers of palau'amide with regard to C37 and C38. Among the NMR spectra of the four synthetic diastereomers, that of 1 was identical to that of natural palau'amide. This established the complete stereostructure. SAR study of aurilide was carried out, and target biomolecule of aurilide was determined to be prohibitin in the mitochondria. Synthetic studies on miuraenamide have been carried out. Miuraenamide induces actin polymerization and stabilizes actin filament. In addition, isolation, structure determination, and synthetic studies of related compounds have been carried out.