通電式計測システムによる微量重金属イオンの特異的分析

研制了一种配备电解装置的流动注射微量重金属测定系统。采用作者独创的基于酶活化的分析方法，实现了利用酶促方法对重金属离子的微量分析。用2 mA的直流电穿过设置在酶柱入口和出口之间的两个管状铂电极，并将其pH控制在等电点上，结果表明，配位于酶活性中心的重金属离子被正向去除，然后酶可以转化为相应的脱辅酶。用电解装置和平面型固定化酶相结合的流通式反应器可以成功地制造出不会因施加直流电而导致终端电压增加的固定化酶。

A flow-injection microdetermination system armed with an electrolytic device for trace amount of heavy metal ions was developed. A unique analytical method based on the apoenzyme activation originally created by the author was applied to realize microanalysis of heavy metal ions with use of enzymatic methods. Use of applications of 2 mA direct current across two tubular platinum electrodes set between an inlet and also outlet of the enzyme column, and also of which pH controlled at the isoelectric point, showed that removal of heavy metal ions coordinated in the active site of the enzymes was positively done and then, the enzymes could be converted to the corresponding apoenzymes. Fabrication of a flow-through reactor armed with an electrolytic device in combination with a planar type of immobilized enzymes, suffering from no increase in terminal voltage due to application of direct currents, could be successfully realized.