The evaluation of plasma-membrane injury and the effect of a moisturizer on injury reduction in freezing of tissue level

本研究利用控制细胞取向和细胞密度的单层培养细胞，研究了在冻融过程中，由于细胞密度较高，细胞在组织水平上的接触状态对质膜损伤的影响。此外，还探讨了保湿剂透明质酸在质膜附近的水合物提高细胞存活率的可行性。结果表明，质膜损伤随着细胞密度的增加而增加，损伤的程度取决于细胞的取向和冷冻顺序。此外，透明质酸对质膜有明显的冷冻保护作用。

In this study, influence of the contact state of cells in the sample of tissue level as high cell density on a plasma-membrane injury in a freeze-thaw process was investigated using the monolayer-culture cell which controlled the orientation and the cell density. Furthermore, feasibility of the improvement in cell viability by the hydrate near the plasma membrane of the hyaluronan which is a moisturizer was investigated. As a result, it was suggested that a plasma-membrane injury increased with increasing the cell density, and that the extent of an injury depended on a cell orientation and freezing sequences. Moreover, it is clear that hyaluronan has cryoprotective effect for plasma-membrane.