Functional Analysis of auxin and microRNA in organ formation during rice embryogenesis

以3个水稻突变体——杆状胚1 （cle1）、杆状胚3 （cle3）、缺色氨酸矮化体（tdd1）为研究对象，研究了生长素和miRNA对水稻胚发生中器官分化的调控作用。两个突变体cle1和cle3的胚胎表型为棒状，而tdd1的胚胎表型为球状；这三个突变体在胚胎发生过程中器官形成有缺陷。定位克隆表明，cle1和tdd1的负责基因分别为OSDCL1和ASB1。详细分析揭示了生长素和miRNA对水稻胚胎发生的重要调控作用。另外两个突变体cre3和tdd1也进行了分析。

The three rice mutants, club-shaped embryo1 (cle1), club-shaped embryo3 (cle3), tryptphan deficient dwarf1 (tdd1) were used to study the regulation for organ differentiation in rice embryogenesis by auxin and miRNA. The embryonic phenotype of two mutants, cle1 and cle3, are club-shaped, and that of tdd1 is globular ; the three mutants are defective for the organ formation during embryogenesis. The positional cloning indicates that the responsible genes for cle1 and tdd1 are OSDCL1 and ASB1, respectively. The detailed analysis revealed the important regulation by auxin and miRNA for rice embryogenesis. The other two mutants, cre3 and tdd1, were also analyzed.