Regulation of unequal cell division in animal oocyte meiosis

在动物卵母细胞的减数分裂成熟过程中，没有DNA复制的中间阶段，两个连续的分裂发生，从而产生单倍体配子。此外，这两个分裂是极不对称的，以确保大多数母体储存在卵母细胞内。这导致形成不同大小的子细胞：大的卵母细胞和小的极体。目前尚不清楚是什么信号将主轴极定位与极体形成相耦合。我们发现，Mos-MAPK-Rsk激酶途径是正常极体挤压所必需的。因此，我们通过分析在极体挤压过程中被激酶途径特异性磷酸化的蛋白质来解决这个问题。我们已经确定了一些候选的调整为主轴杆定位。现在，我们正在检查每个候选细胞在不对称细胞分裂中扮演的角色。

During meiotic maturation of animal oocytes, two successive divisions occur without an intermediate phase of DNA replication, so that haploid gametes are produced. Moreover, these two divisions are extremely asymmetric, to ensure that most of the maternal stores are retained within the oocyte. This leads to the formation of daughter cells with different sizes : the large oocyte and the small polar bodies. It is not known what signal couples the spindle pole positioning to polar body formation. We have found that Mos-MAPK-Rsk kinase pathway is required for the normal polarbody extrusion. So, we approached the question by analyzing the proteins that are phosphorylated specifically by the kinase pathway during the polarbody extrusion. We have identified some candidates for the regulators for the spindle pole positioning. Now, we are checking each candidate play a role in the asymmetric cell division.