Unicellular diazotrophic cyanobacteira : development of immunocytochemical methods for nitrogenase detection and analysis of their role in Kuroshio Waters.

发展了适用于检测海洋沃茨中单细胞重氮蓝藻的免疫细胞化学方法。本研究以黑潮沃茨（爱媛县外海、宫城县外海及台湾南部外海）为研究对象，以单细胞重氮蓝细菌为研究对象。单细胞固氮蓝藻的检测也尝试使用固氮酶和16 SrRNA基因的DNA扩增。在爱媛县和宫城外的沃茨中，通过免疫细胞化学和基于DNA的方法检测到少量固氮单细胞蓝藻。台湾外海沃茨中有大量的单细胞固氮蓝细菌，细胞密度为0.5 ~ 1.5×103/L。从爱媛近海（UWA 1）和台湾（TW 3）成功分离到两株固氮蓝藻。鉴定为粘杆藻属（Gloeothece spp.）（UWA 1）和蓝杆藻属（Cyanothece spp.）(TW3)从16 SrRNA和固氮酶（nifH）基因的部分序列及其形态学特征分析，初步确定了该菌的形态学特征。

Immunocytochemical methods that is applicable for detection of unicellular diazotrophic cyanobacteria in sea waters have been developed. Using developed methods, unicellular diazotrophic cyanobacteria were detected in sea waters collected from Kuroshio waters (off Ehime Prefecture, off Miyagi prefecture and off from South Taiwan). Detection of unicellular diazotrophic cyanobacteria was also tried using DNA-amplification of nitrogenase and 16SrRNA genes. Few diazotrophic unicellular cyanobacteria were detected by both immunocytochemical and DNA-based methods in waters from off Ehime and Miyagi. Unicellular diazotrophic cyanobacteria was abundant in waters from off Taiwan : cell densities were 0.5 to 1.5×103/L. Two diazotrophic cyanobacteria were successfully isolated from off Ehime (UWA1) and Taiwan (TW3). They were identified as Gloeothece spp. (UWA1) and Cyanothece spp. (TW3) from the partial sequences of 16S rRNA and nitrogenase (nifH) genes and their morphology.