Species diversity of pasture grass Skeletonema in the world sea and assessment of its classification

2007年10月和2008年1月至2009年12月，通过形态学扫描电镜观察和分子生物学分析（主要是大亚基（LSU）rDNA），在日本东海湾工业港的一个监测站发现了7种螺旋藻。我们使用LSU rDNA将一个物种鉴定为S.dohrnii，但使用小亚基（SSU）rDNA将其鉴定为S.marinoi，因此我们使用术语S。马里诺-多尔尼复合体这是S.ardens、S.肋突s.s.，和假中肋骨条藻（S.pseudocostatum）在日本已被记录，而门氏链霉菌（S.menzelii）和热带链霉菌（S.tropicum）在日本已被分子生物学方法鉴定。色葡萄海洋-杜氏复合体全年分离频率较高，日本血吸虫也有较高的分离频率，但夏季分离频率较低。热带链霉菌在9 ~ 12月分离到，而雅顿链霉菌和热带链霉菌在9 ~ 12月分离到。肋骨只有在水温超过20℃时才能分离。S.pseudocostatum只在夏季开花一次，但S.menzelii在5月和10月再次分离。S.第一肋骨节是由这七个物种的演替所支撑的。除S. menzelii，是该湾藻华的重要组成部分。每个月分离到1 ~ 4种螺旋藻。月物种多样性以中肋骨条藻、中肋骨条藻、和S.tropicum通常与热带或亚热带沃茨，被分离。总的来说，独开湾的棘丝藻属物种多样性很高。

Seven Skeletonema species were identified at one station in the industrial harbor Dokai Bay, Japan, in October 2007 and then monthly from January 2008 to December 2009 by morphological scanning electron microscopy observations and molecular analyses of mainly the large subunit (LSU) rDNA. We refer to one species identified as S.dohrnii using LSU rDNA, but as S.marinoi using the small subunit (SSU) rDNA and consequently we use the term S. marinoi-dohrnii complex. This is the first time that S.ardens, S. costatum s.s., and S.pseudocostatum have been recorded in Japan and that S.menzelii and S.tropicum have been identified by molecular methods in Japan. The S. marinoi-dohrnii complex was isolated with high frequency all year-round, and S.japonicum was also isolated with high frequency but not so in summer. S.tropicum was isolated from September to December, but S.ardens and S. costatum s.s. were isolated only when the water temperature exceeded 20℃. S.pseudocostatum bloomed just once, in summer, but S.menzelii was isolated in May and again in October. The continuous year-round occurrence of S. costatum s.1. in the bay is supported by the succession of these seven species. Six of the species, except S. menzelii, were important components of algal blooms in the bay. One to four species of Skeletonema were isolated every month. Monthly species diversity was higher when S.ardens, S.costatum s.s., and S.tropicum that are usually associated with tropical or subtropical waters, were isolated. In general, species diversity of the genus Skeletonema was very high in Dokai Bay.