Search of new therapeutic agents for drug dependence and analysis of the inherited difference in patients with drug dependence.

我们研究了l型高压门控钙通道在心理和生理依赖中功能增强的详细和新的共同机制。在持续乙醇处理的神经细胞中观察到热休克因子的磷酸化和随之而来的热休克蛋白70 （HSP70）的增加。此外，长期暴露于乙醇后，细胞内亚铁浓度也显著升高。这些结果提示细胞内HSP70表达和亚铁浓度的升高可能是药物依赖机制的新靶点。

We investigated the detailed and new common mechanisms underling the accentuation of L-type high voltage-gated calcium channels function in establishment of psychological and physical dependence. Phosphorylation of heat shock factor and following increase in heat shock proteins 70 (HSP70) were observed in sustained ethanol-treated neuronal cells. In addition, intracellular ferrous iron concentrations were also significant increased after long-term exposure of ethanol. These results suggest the possibility that increase in intracellular HSP70 expressions and ferrous iron concentrations are new target for mechanism of drug dependence.