Gene expression profiling of bone-marrow derived diffuse large B-cell lymphoma

对29例以骨髓为首发表现的弥漫性大b细胞淋巴瘤（BM-DLBCL）进行V_H基因序列分析，并进行趋化因子及趋化因子受体基因芯片表达及免疫组化分析。对18例病例进行PCR和测序分析，发现V_H3-7、V_H4-34和V_H4-39是常见的V_H基因，VH3-48、VH3-23、VH3-21等18个V_H基因中有14个与自身免疫性疾病相关。此外，趋化因子和趋化因子受体特异性的cDNA芯片分析显示，趋化因子受体XCR1在BM-DLBCL病例中的表达显著升高（P< 0.05），定量逆转录- pcr和免疫组织化学分析证实了这一点。趋化因子受体XCR1的表达和自身反应性V_H基因的频繁使用似乎是BM-DLBCL的明显特征。我是J·克林·帕索尔，新闻报道。

A total of 29 cases of diffuse large B-cell lymphoma initially manifesting in the bone marrow (BM-DLBCL) were analyzed for V_H gene sequence, and expression microarray of chemokines and chemokine receptors and immunohistochemical analysis were done. Seminested polymerase chain reaction (PCR) and sequencing analyses of 18 cases revealed that V_H3-7, V_H4-34, and V_H4-39 were frequently used, and the V_H gene usage in 14 of 18 V_H genes including VH3-48, VH3-23, VH3-21 were those associated with autoimmune diseases. Furthermore, cDNA microarray analysis specific for chemokine and chemokine receptors revealed that chemokine receptor XCR1 expression was significantly elevated in the BM-DLBCL cases (P<.05), which was confirmed by quantitative reverse transcriptase-PCR and immunohistochemical analysis. Expression of the chemokine receptor XCR1 and frequent usage of autoreactive V_H genes seem to be distinct characteristics of BM-DLBCL. Am J Clin Pathol, In press.