Analysis of the molecular mechanism of the quantitative resistance to grapevine anthracnose.

通过改变培养基的浓度，建立了一种生产足量黑桃分生孢子的可靠方法。该方法可方便、稳定地评价葡萄对炭疽病的抗性。对94株葡萄的抗性进行了评估，并通过其病变大小有效地显示了每株葡萄的抗性。抗性葡萄在真菌入侵后不久就出现了超敏反应和棕色化合物（酚类）的积累，并且进一步侵入植物细胞受到限制。结果表明，中抗葡萄品种对黑线葡萄的抗性机制基本相同。

A reliable method to produce sufficient amount of conidia of Elsinoe ampelina was developed by modifying the concentration of the media. It enables us to evaluate the resistance of grapevines to anthracnose easily and stably. The resistance of 94 grapevines was evaluated, and the resistance of each vine was effectively shown by their lesion sizes. The hypersensitive response and the accumulation of brown compounds (phenolics) were observed soon after the invasion of the fungus in the resistant grapevine, and the further invasion into the plant cells was restricted. It was implied that the moderate resistant Vitis vinifera cultivars might have almost same mechanism against E. ampelina.