functional analyses of the intracellular phospholipase A1 family in testes

磷脂酶A1（PLA 1）从二酰基甘油磷脂中裂解sn-1脂肪酰基，产生游离脂肪酸和sn-2-溶血磷脂。细胞内磷脂酶A1（2009年命名为iPLA 1）家族由α、β、γ三个成员组成。为了揭示iPLA 1家族成员的生理功能，采用基因打靶技术建立了iPLA 1-β，-γ基因敲除小鼠（KO）。iPLA 1-β和-γKO小鼠出生，并且显然与野生型小鼠无法区分。然而，雄性iPLA 1-β KO小鼠生育力低下。

Phospholipases of the A1 (PLA1) cleave the sn-1 fatty acyl group from diacyl glycero-phospholipids and produce a free fatty acid and an sn-2-lysophospholipid. The intracellular phospholipase A1 (named iPLA1 from 2009 ) family consists of three members,α,β,γ. To reveal the physiological functions of iPLA1 family member, iPLA1 -β,-γknockout mice(KO)were generated by gene targeting. iPLA1-β and -γKO mice were born and were apparently indistinguishable from wild-type mice. However, male iPLA1-β KO mice were subfertile.