An orally applicable Shiga toxin neutralizer functions in the intestine to inhibit the intracellular transport of the toxin.

我们发现PPP-tet，一种基于肽的Stx2抑制剂，完全抑制直接注射Stx2引起的家兔回肠积液。我们还发现PPP-tet仅通过与Stx2形成复合物而在回肠上皮细胞中积累。培养上皮细胞中的复合物阻断了Stx2从高尔基体到内质网的细胞内转运，而这一过程是Stx2细胞毒性的必要过程。我们的研究结果表明，PPP-tet通过改变上皮细胞中Stx2的细胞内转运，在肠道中作为一种有效的Stx中和剂发挥作用。

We found that PPP-tet, a peptide-based Stx2 inhibitor, completely inhibited fluid accumulation in the rabbit ileum caused by the direct injection of Stx2. We also found that PPP-tet accumulated in the ileal epithelial cells only through its formation of a complex with Stx2. The complex in cultured epithelial cells blocked the intracellular transport of Stx2 from the Golgi apparatus to the endoplasmic reticulum, the process that is essential for Stx2 cytotoxicity. Our results reveal that PPP-tet functions as a potent Stx neutralizer in the intestine by altering the intracellular transport of Stx2 in epithelial cells.