IDENTIFICATION OF KEY FACTORS IN CISPLATIN-INDUCED NEPHROTOXICITY BY MICROARRAY ANALYSIS USING THE ISOLATED RENAL TUBULES

肾脏由几个肾段组成。为了阐明肾小管中的基因表达谱，这是顺铂诱导的肾毒性的起源，使用分离的肾小管进行微阵列分析。首先，我们证实了这种实验方法反映了肾小管基因的表达。在顺铂处理的大鼠的离体肾小管中，趋化因子的基因表达升高。趋化因子可能是顺铂肾毒性中肾小管基因的关键因素。

Kidney consists of several renal segments. To clarify the gene expression profile in the renal tubule, which is the origin of cisplatin-induced nephrotoxicity, microarray analysis using the isolated tubules was carried out. At first, we confirmed that this experimental method reflected the tubular gene expression. In the isolated tubules of cisplatin-treated rats, the gene expression of chemokines was elevated. Chemokines are suggested to be the key factors of the renal tubular genes in cisplatin-induced nephrotoxicity.