Functional analysis of miRNA dependent cisplatin resistance mechanisms.

顺铂是一种有效的抗癌药物，广泛应用于癌症的治疗。然而，顺铂耐药性的发展是治疗的主要障碍。为了了解临床相关的耐药机制，许多研究旨在阐明顺铂耐药细胞的生化/分子改变。然而，这些研究并没有最终确定细胞对顺铂耐药的基础。本研究采用芯片技术和实时RT-PCR技术分析顺铂耐药细胞系的微RNA表达谱。我们发现一些微rna在一对顺铂耐药的非小肺癌细胞系中受到类似的调节。这些发现为理解miRNA介导的顺铂耐药机制提供了有用的信息。

Cisplatin is an effective anticancer agent that is widely used in the treatment of cancer. However, the development of resistance to cisplatin is a major obstacle to treatment. To understand the clinically relevant mechanisms of resistance, many studies have been aimed at clarifying the biochemical/molecular alterations of cisplatin-resistance cells. However, these studies did not conclusively identify the basis of cellular resistance to cisplatin.In this study, micro RNA expression profiles of cisplatin resistant cell lines were analyzed using microarray and real time RT-PCR. We found some of micro RNAs were similarly regulated in couple of cisplatin resistant non small lung cancer cell lines. These finding were useful information for understanding of the miRNA mediated cisplatin resistance mechanisms.